Download or read book Osteoblast Cell Response to Titanium Dental Implant Surfaces Treated with Different Chemical Agents written by Masa Roland and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Osteoblast cell response to titanium dental implant surfaces treated with different chemical agentsAbstractRelatively high survival rates of dental implants encourage clinicians to use this type of oral rehabilitation frequently in their daily practice. However, implant failures are increasing parallel with the number of implant placements. Bacterial colonization is one of the main causes of periimplant inflammation. In the therapy of both periimplant mucositis and periimplantitis mechanical debridement and chemical decontamination are fundamental steps.1 In order to be up to date in the fight against bacteria, various types of antibacterial solutions should be used during pocket irrigation. However, they should undergo through different in vitro and in vivo tests before daily use. In this study the response of primary osteoblast cells to chemically treated titanium surfaces was examined. Different chemical and cell viability tests, and for visualization fluorescent staining were carried out.Background In case of peri-implant infections, the main goals of treatment are to decontaminate the dental implant surface in order to reduce the bacterial load and to facilitate reintegration. Various chemical agents have been proposed for surface decontamination in the therapy of peri-implantitis. However these antibacterial agents could alter the surface properties of the implant material and also the osteoblast response.Aim/HypothesisOur aim was to evaluate the interaction of titanium dental implant surface with three different antibacterial solutions: chlorhexidine, povidone-iodine and chlorine dioxide. Our null hypothesis was that residues of these agents do not modify either the implant surface or the osteoblast response.Materials and MethodsCommercially pure (CP4) sandblasted, acid-etched titanium sample discs (Dentiu00ae System Ltd., Hungary) 1.5 mm thick and 9 mm in diameter, rinsed with ultrapure water, were used as control surfaces. Three different chemical agents were investigated: chlorhexidine digluconate (Curasept ADS 220, 0.2%, Switzerland), povidone-iodine (Betadine, 10 %, Switzerland) and chlorine dioxid solution (Solumium, 0.12%, Hungary). Titanium sample discs were treated for 5 minutes in each case. Wettability studies were performed by an OCA 20 (Dataphysics, Filderstadt, Germany) contact angle measuring device with water and diiodomethane drops. Surface free energy (SFE) was determined according to Owen-Wendt-Rabel-Kaelble (OWRK) method. The chemical compositions of the Ti discs were studied by X-ray photoelectron spectroscopy (XPS). In vitro attachment (24h) and proliferation (72h) of primary osteoblast cells were investigated via dimethylthiazol- diphenyltetrazolium bromide (MTT) and fluorescence microscopy.ResultsContact angle measurements revealed significant differences in SFE of the treated discs compared to the control Ti surface. Povidone-iodine treated samples had the lowest contact angles and the highest SFE. Chlorhexidine (CHX) - and chlorine dioxide (ClO2) - treated discs showed significantly higher contact angle values compared to control Ti samples. XPS confirmed the constant presence of typical surface elements (Ti, O,C,N) on each surface, while chlorine was detected only in the ClO2 group. MTT test proved the viability of primary osteoblast cells after chemical agent treatment. In spite of the significant difference in surface free energy, and the chlorine presence in the ClO2 group, MTT assay indicated similar osteoblast cell attachment (24h) in the three study groups and the control groups (plate and Ti). After 72h, a significant increase was observed in cell numbers (high proliferation rate) in all of the investigated groups compared to cell numbers after 24h, no significant difference was found beetwen the groups. Fluorescent images confirmed these results. Conclusion and Clinical implicationsIn spite of the different wettabilities, primary osteoblast cells showed similar response and high viability on each of the surfaces. Therefore, it seems no far-fetched to assume that none of the irrigating agents would have a negative influence on osseointegration. In conclusion, we suggest the use of PVPI and high purity ClO2-solution beside the gold standard CHX as implant surface decontaminating agents, in order to reach broader antibacterial efficiency. Key words: peri-implantitis, chlorhexidine, povidone-iodine, chlorine dioxide, osteoblast, titanium dental implantAcknowledgmentsThe work was supported by the grant GINOP-2.3.2-15-2016-00011 to a consortium led by the University of Szeged, Szeged, Hungary (participants: the University of Debrecen, Debrecen, and the Biological Research Centre, Hungarian Academy of Sciences, Szeged, Hungary).