Download or read book Rapid Detection and Characterization of Foodborne Pathogens by Molecular Techniques written by Robert E. Levin and published by CRC Press. This book was released on 2009-10-26 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by one of the most prolific and respected researchers in food safety, this volume describes molecular techniques for the detection and discrimination of major infectious bacteria associated with foods. Each chapter deals with a specific organism and techniques applied to that organism. Particular focus is placed on genes associated with pathogenicity used in the polymerase chain reaction (PCR) including real-time PCR for specific detection of pathogenic bacteria and the inherent limitations of such methodology with certain pathogens. Methods for extracting microorganisms from complex food matrices andDNA purification techniques are also emphasized.