Download or read book Enhancing Bone Marrow-Derived Mesenchymal Stem Cell Therapeutic Potential Through MTOR Inhibition written by Lisa M. Stukenborg and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Despite our best efforts to avoid growing older, aging is an inevitable phenomenon that affects all living organisms. The aging process results from normal biological processes that are necessary for maintaining cellular homeostasis, including protein translation and regulation, lipid and nucleotide biosynthesis, nuclear maintenance and chromatin modifications. Over time, cells begin to accumulate damage, enter a unique state known as senescence, disrupting their functionality and thereby contributing to a reduction in organismal lifespan. One of the hallmarks of senescence is an irreversible arrest of the cell cycle resulting in cells which are no longer able to proliferate. Since its discovery, senescence in somatic cells has been well defined, but there are still limitations of our knowledge regarding senescence in stem cells, however, a progressive decline in adult stem cell function is a feature of aged tissues and contributes to multiple age-related diseases. Of particular interest are bone marrow mesenchymal stem cells, also known as bone marrow stromal cells (BMSCs), which are multipotent and have significant therapeutic potential. In this study, we aimed to understand senescence in human bone-marrow mesenchymal stem cells. In addition, we examined the impact of inhibiting the mechanistic/mammalian target of rapamycin (mTOR), using rapamycin, on senescence in BMSCs. We found that stem cells undergo senescence similarly to somatic cells, but also have unique features that have not yet been reported. Due to the implications of senescence in various diseases, this research helps shed light on the role of stem cells during organismal aging.