Fed-Batch Fermentation

Fed-Batch Fermentation
Author :
Publisher : Woodhead Publishing
Total Pages : 0
Release :
ISBN-10 : 1907568921
ISBN-13 : 9781907568923
Rating : 4/5 (923 Downloads)

Book Synopsis Fed-Batch Fermentation by : G G Moulton

Download or read book Fed-Batch Fermentation written by G G Moulton and published by Woodhead Publishing. This book was released on 2014-09-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fed-batch Fermentation is primarily a practical guide for recombinant protein production in E. coli using a Fed-batch Fermentation process. Ideal users of this guide are teaching labs and R&D labs that need a quick and reproducible process for recombinant protein production. It may also be used as a template for the production of recombinant protein product for use in clinical trials. The guide highlights a method whereby a medium cell density - final Ods = 30-40 (A600) - Fed-batch Fermentation process can be accomplished within a single day with minimal supervision. This process can also be done on a small (2L) scale that is scalable to 30L or more. All reagents (media, carbon source, plasmid vector and host cell) used are widely available and are relatively inexpensive. This method has been used to produce three different protein products following cGMP guidelines for Phase I clinical studies.


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