Download or read book Robotic Single Cell Manipulation for Biological and Clinical Applications written by Clement Leung and published by . This book was released on 2011 with total page 198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single cell manipulation techniques have important applications in laboratory and clinical procedures such as intracytoplasmic sperm injection (ICSI) and polar body biopsy for preimplantation genetic diagnosis (PGD). Conventionally, manipulation of cells conducted in these procedures have been performed manually, which entails long training hours and stringent skills. Conventional single cell manipulation also has the limitation of low success rates and poor reproducibility due to human fatigue and skill variations across operators. This research focuses on the integration of computer vision microscopy and control algorithms into a system for the automation of the following single cell manipulation techniques: (1) sperm immobilization, (2) cell aspiration into a micropipette, and cell positioning inside a micropipette, and (3) rotational control of cells in three-dimensions. These automated techniques eliminate the need for significant human involvement and long training. Through experimental trials on live cells, the automated techniques demonstrated high success rates.